NEURONAL TRACING BY ELECTROCHEMICALLY CONTROLLED IDENTIFICATION OF TARGETS
Relevant for Research Area
Dr. Maria Asplund (Contact PI)
Most neural probes have to be explanted before histological analysis can be made which is problematic when electrophysiological information is to be correlated to histological findings. In retrospect, it can be close to impossible to reconstruct exactly which group of neurons that were monitored or stimulated. We here develop a concept for equipping electrodes with staining functionality. PEDOT electrodes can be loaded with fluorescent dye to be released, and leave a fluorescent footprint in tissue, at the study end point. This strengthens the possibilities to study neurological diseases by combined electrophysiology and histology and furthermore can shed light on the interaction between electrical stimulation and tissue response. By using implantable pH sensors we will continuously monitor metabolic reactions surrounding the electrode which can be further analyzed histologically based on our staining electrode technology. The interplay between stimulation, local pH changes and adverse reactions will thereby be analyzed.
We have developed electrodes which are excellent for intracranial recordings and stimulation and in addition carry a built-in on-demand labelling functionality controlled by an electrochemical driving signal. Our electrodes thereby can contribute to stronger analysis and improved understanding of how neurological diseases develop over time based on combined electrophysiology and histology.